Demineralization and Hydrogen Peroxide Penetration in Teeth with Incipient Lesions

Autoři: André Luiz Fraga Briso 1;  Rafael Simões Gonçalves 1;  Fernanda Bernardi Da Costa 1;  Marjorie De Oliveira Gallinari 1;  Luciano Tavares Angelo Cintra 1;  Paulo ;  Henrique Dos Santos 2
Působiště autorů: Department of Restorative Dentistry, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Araçatuba, SP, Brazil 1;  Department of Dental Materials and Prosthodontics, Araçatuba Dental School, UNESP - Univ Estadual Paulista, Araçatuba, SP, Brazil 2
Vyšlo v časopise: Brazilian Dental Journal (2015) 26(2)
Kategorie: Original articles
doi: 10.1590/0103-6440201300225

This is an open-access article distributed under the terms of the Creative Commons Attribution License.
The electronic version of this article is the complete one and can be found online at:


The aim of this study was to evaluate the demineralization and hydrogen peroxide (HP) penetration in teeth with incipient lesions submitted to bleaching treatment. For analysis of HP penetration, sound and demineralized enamel/dentin discs were placed in artificial pulp chambers containing acetate buffer solution. After bleaching treatment, this solution was subjected for analysis of optical density by spectrophotometry and the disc surfaces were analyzed with scanning electron microscopy (SEM) and polarized light microscopy (PLM). The remaining discs were subjected for cross-sectional hardness analysis at different depths. Data were analyzed by repeated measures ANOVA and PLSD Fisher test (a=0.05). It was observed that previously demineralized teeth showed greater HP penetration (p<0.05). The bleaching treatment caused changes to a depth of 20 µm in sound enamel and up to 90 µm in demineralized enamel. SEM and PLM images revealed that the bleaching treatment caused superficial changes that were considerably more accentuated in previously demineralized teeth. It may be concluded that the enamel mineralization level influences HP penetration and the bleaching agent contributed to increase the demineralization depth.

Key words:
bleaching; hydrogen peroxide; tooth demineralization; caries


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